|
|
Registro Completo |
Biblioteca(s): |
Embrapa Café. |
Data corrente: |
15/12/2017 |
Data da última atualização: |
15/12/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BARKA, G. D.; CAIXETA, E. T.; ALMEIDA, R. F. de; ALVARENGA, S. M.; ZAMBOLIM, L. |
Afiliação: |
Geleta Dugassa Barka, BIOCAFÉ/BIOAGRO/UFV; EVELINE TEIXEIRA CAIXETA, SAPC; Robson Ferreira de Almeida, Instituto Federal do Espírito Santo; Samuel Mazzinghy Alvarenga, FEPAGRO; Laércio Zambolim, Departamento de Fitopatologia/Universidade Federal de Viçosa. |
Título: |
Differential expression of molecular rust resistance components have distinctive profiles in Coffea arabica - Hemileia vastatrix interactions. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
European Journal of Plant Pathology, v. 149, n. 3, p. 543-561, Nov. 2017. |
Idioma: |
Inglês |
Conteúdo: |
Countering the economic hurdle caused by coffee leaf rust disease is most appealing at this time as it has posed a major threat to coffee production around the world. Establishing differential expression profiles at different times following pathogen invasion in both innate and acquired immunities unlocks the molecular components of resistance and susceptibility. Suppression subtractive hybridization (SSH) was used to identify genes differentially over-expressed and repressed during incompatible and compatible interactions between Coffea arabica and Hemileia vastatrix. From 433 clones of expressed sequence tags (ESTs) sequenced, 352 were annotated and categorized of which the proportion of genes expressed during compatible interaction were relatively smaller. The result showed upregulation and downregulation of various genes at 12 and 24 h after pathogen inoculation in both interactions. The use of four different databases in searching for gene homology resulted in different number of similar sequences. BLASTx against EMBL-EBI (European Molecular Biology Laboratory-European Bioinformatics Institute) database being with the maximum (100%) hits for all the annotated sequences. RT-qPCR analysis of seven resistance-signaling genes showed similar expression patterns for most of the genes in both interactions, indicating these genes are involved in basal (nonspecific) defense during which immune reactions are similar. Using SSH, we identified different types of resistance related genes that could be used for further studies towards resistant cultivar development. The potential role of some of the resistance related proteins found were also discussed. MenosCountering the economic hurdle caused by coffee leaf rust disease is most appealing at this time as it has posed a major threat to coffee production around the world. Establishing differential expression profiles at different times following pathogen invasion in both innate and acquired immunities unlocks the molecular components of resistance and susceptibility. Suppression subtractive hybridization (SSH) was used to identify genes differentially over-expressed and repressed during incompatible and compatible interactions between Coffea arabica and Hemileia vastatrix. From 433 clones of expressed sequence tags (ESTs) sequenced, 352 were annotated and categorized of which the proportion of genes expressed during compatible interaction were relatively smaller. The result showed upregulation and downregulation of various genes at 12 and 24 h after pathogen inoculation in both interactions. The use of four different databases in searching for gene homology resulted in different number of similar sequences. BLASTx against EMBL-EBI (European Molecular Biology Laboratory-European Bioinformatics Institute) database being with the maximum (100%) hits for all the annotated sequences. RT-qPCR analysis of seven resistance-signaling genes showed similar expression patterns for most of the genes in both interactions, indicating these genes are involved in basal (nonspecific) defense during which immune reactions are similar. Using SSH, we identified different types of resistance related ... Mostrar Tudo |
Palavras-Chave: |
Coffee; Plant-pathogen interaction; Rust disease. |
Thesagro: |
Café; Ferrugem; Patogeno. |
Thesaurus Nal: |
Gene expression; pest resistance. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/169104/1/Differential-expression-of-molecular-rust-resistance.pdf
|
Marc: |
LEADER 02491naa a2200265 a 4500 001 2082676 005 2017-12-15 008 2017 bl uuuu u00u1 u #d 100 1 $aBARKA, G. D. 245 $aDifferential expression of molecular rust resistance components have distinctive profiles in Coffea arabica - Hemileia vastatrix interactions.$h[electronic resource] 260 $c2017 520 $aCountering the economic hurdle caused by coffee leaf rust disease is most appealing at this time as it has posed a major threat to coffee production around the world. Establishing differential expression profiles at different times following pathogen invasion in both innate and acquired immunities unlocks the molecular components of resistance and susceptibility. Suppression subtractive hybridization (SSH) was used to identify genes differentially over-expressed and repressed during incompatible and compatible interactions between Coffea arabica and Hemileia vastatrix. From 433 clones of expressed sequence tags (ESTs) sequenced, 352 were annotated and categorized of which the proportion of genes expressed during compatible interaction were relatively smaller. The result showed upregulation and downregulation of various genes at 12 and 24 h after pathogen inoculation in both interactions. The use of four different databases in searching for gene homology resulted in different number of similar sequences. BLASTx against EMBL-EBI (European Molecular Biology Laboratory-European Bioinformatics Institute) database being with the maximum (100%) hits for all the annotated sequences. RT-qPCR analysis of seven resistance-signaling genes showed similar expression patterns for most of the genes in both interactions, indicating these genes are involved in basal (nonspecific) defense during which immune reactions are similar. Using SSH, we identified different types of resistance related genes that could be used for further studies towards resistant cultivar development. The potential role of some of the resistance related proteins found were also discussed. 650 $aGene expression 650 $apest resistance 650 $aCafé 650 $aFerrugem 650 $aPatogeno 653 $aCoffee 653 $aPlant-pathogen interaction 653 $aRust disease 700 1 $aCAIXETA, E. T. 700 1 $aALMEIDA, R. F. de 700 1 $aALVARENGA, S. M. 700 1 $aZAMBOLIM, L. 773 $tEuropean Journal of Plant Pathology$gv. 149, n. 3, p. 543-561, Nov. 2017.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Café (CNPCa) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Algodão. Para informações adicionais entre em contato com cnpa.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Algodão. |
Data corrente: |
28/05/2009 |
Data da última atualização: |
28/05/2009 |
Autoria: |
VENTURIERI, A. (Ed.). |
Afiliação: |
Adriano Venturieri, CPATU. |
Título: |
Zoneamento ecológico-econômico da área de influência da Rodovia BR-163 (Cuiabá-Santarém) : gestão territorial. |
Ano de publicação: |
2007 |
Fonte/Imprenta: |
Belém, PA: Embrapa Amazônia Oriental, 2007. |
Volume: |
v. 3. |
Páginas: |
101 p. |
Descrição Física: |
il. |
ISBN: |
978-85-87690-64-7 |
Idioma: |
Português |
Notas: |
Acompanha 5 mapas. |
Palavras-Chave: |
Brasil; Cuiabá; Desenvolvimento regional; Gestão territorial; Mato Grosso; Pará; Planejamento ambiental; Santarém; Zoneamento econômico. |
Thesagro: |
Biodiversidade; Ecologia; Economia; Políticas Públicas; Proteção Ambiental; Recurso Natural; Zoneamento Ecológico. |
Thesaurus NAL: |
Amazonia. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01006nam a2200349 a 4500 001 1278361 005 2009-05-28 008 2007 bl uuuu 00u1 u #d 020 $a978-85-87690-64-7 100 1 $aVENTURIERI, A. (Ed.). 245 $aZoneamento ecológico-econômico da área de influência da Rodovia BR-163 (Cuiabá-Santarém)$bgestão territorial. 260 $aBelém, PA: Embrapa Amazônia Oriental$c2007 300 $a101 p. v. 3.$cil. 490 $vv. 3. 500 $aAcompanha 5 mapas. 650 $aAmazonia 650 $aBiodiversidade 650 $aEcologia 650 $aEconomia 650 $aPolíticas Públicas 650 $aProteção Ambiental 650 $aRecurso Natural 650 $aZoneamento Ecológico 653 $aBrasil 653 $aCuiabá 653 $aDesenvolvimento regional 653 $aGestão territorial 653 $aMato Grosso 653 $aPará 653 $aPlanejamento ambiental 653 $aSantarém 653 $aZoneamento econômico
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Algodão (CNPA) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Expressão de busca inválida. Verifique!!! |
|
|